EFFICACY OF DIET SUPPLEMENTATION WITH VITAMIN D AND OMEGA-3 ON RATS WITH INDUCED CROHN’S DISEASE

ABSTRACT
Crohn's disease is an irreversible inflammatory disease, mainly affecting the gastrointestinal tract. The aim of present study was to investigate the effect of dietary vitamin D and omega 3 supplementation on rats with induced crohn's disease. Thirty adult male albino rats weighing (160 gm) were divided into 5 groups. The 1^st group (n=6) was fed on the basal diet. The 2^nd to 5^th of rats ( n=24) were injected with (10 mg/kg for 3 days) of indomethacin to induce Crohn's disease. Groups (3 to 5) were fed on basal diet and given orally 1000/IU/kg/bw of vitamin D,  600 mg/kg/bw of omega 3 and combination of 1000/IU/kg/ bw of vitamin D and 600 mg/kg/bw of omega 3, respectively. At the end of the experimental period (8 weeks), rats were scarified. Biochemical analysis of the tested parameters was carried out.
Results indicated that indomethacin Crohn's rats showed a significant (P< 0.05) decrease in serum albumin, catalase and glutathione peroxidase compared to the negative control one, while serum liver function , MDA ,CRP, and  immunity proteins were significantly increased.
Results showed that Crohn’s rats given vitamin D had significant increased serum vitamin D concentration compared to positive control group .On the other hand, it was observed that, the diet supplemented with omega 3 caused no significant difference in the serum concentration of vitamin D compared to the positive control group .The highest increase in serum vitamin D concentration was observed at the group of rats fed on omega 3 and vtamin D.
It was also observed that supplementation of rats with vitamin D and/or omega 3 at the tested level improved BWG%, liver functions, immunoglobulins and antioxidant activity of Crohn's rats as compared to the positive control group. Also an  improved of intestinal segment of ileum tissue was recorded as well. It could be concluded that combination with vitamin D and omega 3 supplementation alleviating the side effect of Crohn's rats. So, these materials may be benefit for Crohn's patients.