IN VITRO KIDNEY STONE FORMATION, ANTIOXIDANT AND ANTICOAGULATION ACTIVITY OF BIOLOGICALLY ACTIVE EXTRACTS AND FRACTIONS OF KAFF MARYAM (ANASTATICA HIEROCHUNTICA L.)

ABSTRACT:
Kaff Maryam (Anastatica hierochuntica L.) is believed to be very useful in Arab countries for treating various health disorders. Traditional medicine has been gaining it is recognized due to its importance in public health and the administration of infections. This work focuses on the identification and characterization of phenolic compound profiles in methanol and ethyl acetate extracts of A. hierochuntica L. High-performance liquid chromatography (HPLC) and gas chromatography (GC MS) were used to identify the different phenolics in both extracts for bioactive organic compounds in methanol extract as the main established of diagnostic tools for these compounds. Individual polyphenolics compounds were evaluated in two different solvent crude extracts using HPLC and the result recorded that major compound detected were E-Vallinic and Ellagic. Also, GC-MS analysis was performed to investigate the bioactive organic compounds  of methanolic extract of A. hierochuntica L ., the results illustrated that, Benzyl nitrile was identified as a major chemical constituent according to the relative abundance followed by Benzene, (isothiocyanatomethyl) respectively. The purpose, of this study was to investigate the kidney stone formation, antioxidant, and anticoagulant activities of A. hierochuntica L extracts. The potential effect of A. hierochuntica L extracts on crystal growth of calcium oxalate monohydrate(kidney stone) and its inhibitive properties were investigated at 37^oC and pH =6.5 ±0.05. In addition, antioxidant and anticoagulation properties of A. hierochuntica L. activity were performed at different concentrations. The present research highlights the chemical composition and properties of A. hierochuntica L. that may be related to its beneficial effect. The present research highlights the chemical composition and properties of A. hierochuntica L. that may be related to its beneficial effect. The results in these article suggest that polyphenolics content might be contribute to the high urolithiasis inhibition, antioxidant, and anticoagulation activity of this plant and establish it as a valuable natural antiurolithiasis, antioxidant, and anticoagulation source applicable in the drug industry.