ABSTRACT
The present work was conducted in order to investigate the effect of explant type (leaf and stem internode) and auxin type (2,4-Dichlorophenoxyacetic acid, 2,4-D and Naphthalene Acetic Acid ,NAA) at different concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg/l) as well as different concentrations (0, 5, 10, 20 and 40 mg/l) of L-phenylalanine(Phe) or tryptophan (Trp) on Verbesina encelioides callus cultures growth and production of secondary metabolites. Obtained results demonstrated that using leaf explant and supplementation MS medium with 0.5 mg/l NAA could enhanced and recorded the ultimate values of callus fresh weight, antioxidant activity (%), total flavonoids, total phenolic compounds contents and yields of Verbesina encelioides callus. Also, when the highest concentration (40 mg/l) of either L-phenylalanine or tryptophan was combined with 0.5 mg/l NAA, the maximum values of the abovementioned parameters were obtained. L-phenylalanine proved to be more effective than tryptophan in this regard.
(2022). SOME FACTORS AFFECTING IN VITRO SECONDARY METABOLITES PRODUCTION FROM VERBESINA ENCELIOIDES CALLUS CULTURES. Egyptian Journal of Applied Science, 37(11), 80-94. doi: 10.21608/ejas.2022.287925
MLA
. "SOME FACTORS AFFECTING IN VITRO SECONDARY METABOLITES PRODUCTION FROM VERBESINA ENCELIOIDES CALLUS CULTURES", Egyptian Journal of Applied Science, 37, 11, 2022, 80-94. doi: 10.21608/ejas.2022.287925
HARVARD
(2022). 'SOME FACTORS AFFECTING IN VITRO SECONDARY METABOLITES PRODUCTION FROM VERBESINA ENCELIOIDES CALLUS CULTURES', Egyptian Journal of Applied Science, 37(11), pp. 80-94. doi: 10.21608/ejas.2022.287925
VANCOUVER
SOME FACTORS AFFECTING IN VITRO SECONDARY METABOLITES PRODUCTION FROM VERBESINA ENCELIOIDES CALLUS CULTURES. Egyptian Journal of Applied Science, 2022; 37(11): 80-94. doi: 10.21608/ejas.2022.287925
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